Sequence-specific nuclease-based genome targeting systems, i.e., TALENs and CRISPR/Cas9, often cause uniform biallelic and heterozygous mutations in diploid organisms. Direct sequencing of PCR products containing such mutations results in superimposed sequencing chromatograms. This tool serves to decode automatically sequencing chromatograms with biallelic, heterozygous, and homozygous mutations into allelic sequences.
For testing the program, two sequencing ab1 files and the corresponding references are provided.
The tool for DNA reverse and complement.
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